Researchers have focused on the behavior of Listeria monocytozens related to biofilm and how they support the perseverance of the pathogen in the food processing environment.
A study led by the Austrian Competition Center for Feed and Food Quality, Safety and Innovation (FFOQSI) found that Listeria Monocytozens can colon the current biofilms and also survive in them.
Microorganisms mainly live in communities protected by an self-made matrix of extracellular polymeric substances (EPS). Within these so -called biofilm, microbial cells show converted phenotypes and gene expression patterns. Self-made normal matrix acts as a protective shield, nutrient source and provides space for genetic exchange.
Listeria monocytozens can survive in the atmosphere of food production for years. Biofilms can provide a niche for long-term survival as they protect against environmental fluctuations and germs.
The author of FFOQsi’s first study, Eva Voglair said: “Our data suggests that Listeria Monocytozens can colonize in multisactis biofilms, which can increase survival for cleanliness and disinfection in food introduction plants and can support firmness Are.”
A study published in the journal Microbiological researchIt was found that the bacterial and matrix composition of biofilms is not quite replaced by the presence of Listeria monocytozens, which indicates listeria skins in the biofilm. This observation can change the view as an passive survivor and a resident of biofilms as an active player in the processing environment on Listeria monocytozens.
Protection by other bacteria
Scientists examined an isolated behavior of Listeria Monocytozens in a multiscturalis biofilm. It consisted of pseudomonous fracgi, brochex thermosphacta and carnobacterium maltaromaticum. Within the meat area, these three possible deteriorating bacteria have often been separated. Listeria was capable of colonizing the biofilm of multispaces within two hours.
The sequence type (ST) 121 selected Listeria monocytozens stress was repeatedly separated from Austrian meat processing feature. It colonized the company’s production line for at least one year. The transcription activity was analyzed along with the structure of the biofilm community and matrix.
The growth of biofilm under the state of the food processing environment was copied on the surface of stainless steel, under a low temperature of 10 ° C (50 ° F), and a limited but repeated but repeatedly using the supply of nutrients. After growing a multisactis biofilm for 24 hours, scientists introduced Listeria monocytose strain and examined biofilm composition and gene expression.
“Listeria Monocytozens colonized the multisactis biofilm and was responsible for 6.4 percent of all biofilm cells after six hours. Transcriptoptomic analysis has shown that 127 genes of Listeria monocytozens were significantly upgraded compared to Inoculum, including genuine -related genes related to mobility, chemotaxis, iron and protein transport, ”said scientists.
The team monitored the development of biofilm in six and 24 hours and seven days. Pseudomonous Fragi remained the major species, while Listeria monocytozens were able to survive in multisitis biofilm accounting for 2.4 percent of the total biofilm cells after seven days.
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